Standard practice for testing for whole complement activation in serum by solid materials 用固體材料測試血漿中整個活性的標(biāo)準(zhǔn)操作規(guī)程
Standard practice for testing for alternative pathway complement activation in serum by solid materials 通過固體材料測試血清中替換途徑互補活化性的標(biāo)準(zhǔn)實施規(guī)程
However , complement activation presents a potential threat to host cells , as inappropriate activation may lead to tissue damage in many diseases 然而,對機(jī)體來說,補體也具有潛在的威脅,其經(jīng)過不適宜的活化后,可以導(dǎo)致機(jī)體多種組織的損傷。
An array of regulatory proteins have been found , which inhibit the formation of central enzymes involved in early stages of the complement activation pathway . these include membrane cofactor protein ( mcp cd46 ) , decay - accelerating factor protein ( daf cd5 5 ) , complement receptor 1 ( cr1 , cd35 ) , as well as cd59 , which inhibits formation of the membrane attack complex during later stages . these regulatory factors are widely expressed and abundant on many cells , and in fluids of reproductive system 目前發(fā)現(xiàn),機(jī)體多種細(xì)胞以及生殖系統(tǒng)的體液中表達(dá)和分泌豐富的補體調(diào)控蛋白,包括作用于補體活化早期階段的cd55 、 cd46 、 cd35和作用于補體活化終末階段的cd59 ,它們分別通過抑制補體活化過程中關(guān)鍵的c3 、 c5轉(zhuǎn)化酶和抑制形成膜攻擊單位,抵抗補體對自身組織細(xì)胞的攻擊。
We focused on the following aspects ; 1 ) we first assayed the expression of complement receptors and complement - associated molecules on distinct subsets of dendritic cells during their development in order to understand the physical basis of the sensitivity of dendritic cells to complement and its split products ; we next studied the effects of complement activation on the survival of dendritic cells during their development ; and finally examined the effects of the whole complement system , focusing on the ability of one of the split products of complement activation , c5a , and its first subcomponent - c1q , to influence chemotaxis of dendritic cells , as well as allo - t cell stimulatory activity of dc 我們通過免疫磁珠分離了兩種人dc前體,即髓系來源的單核細(xì)胞( monocytes , mo )和淋巴系來源的漿細(xì)胞樣dc ( plasmaeytoiddendriticeells , pdc ) ,對這兩個不同dc亞群進(jìn)行體外誘導(dǎo)培養(yǎng),使其處于不同的分化發(fā)育階段,然后檢測了其表達(dá)補體受體一cd35 ( cri ) 、 cd21 ( crz ) 、 cdilb ( cr3 ) 、 cdlle ( cr4 ) ,補體調(diào)控蛋白一cd46 、 cd55 、以及部分補體片斷分子受體一c3ar 、 csar 、 clqrp的水平。